Composite

Part:BBa_K5416060:Design

Designed by: Jixiao Wu   Group: iGEM24_Imperial-College   (2024-09-28)


pT7LacO + P22 + HRT2-SP + T7 term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1354
    Illegal AgeI site found at 507
    Illegal AgeI site found at 1755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 625


Design Notes

Codon optimized for E. coli. Two ribosome binding sites in this part are also optimized and standardized for translation in E. coli strain K-12


Source

Built from parts BBa_K1624002; BBa_K3187021; BBa_K5416000; BBa_K3187017; BBa_K731721

References